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REMOVAL OF SALT FROM AQUEOUS SOLUTIONS FOR METABOLOMICS: TARGETED SALT PRECIPITATION
REMOVAL OF SALT FROM AQUEOUS SOLUTIONS FOR METABOLOMICS: TARGETED SALT PRECIPITATION
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机译:从代谢组学的水溶液中去除盐:目标盐的沉淀
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摘要
Methods for removing salts from a metabolite solution. The methods comprise forming an insoluble silver phosphate salt. Further methods include methods for removing hydrolyzed fluorous compounds. These methods comprise extraction with a fluorous solvent in the presence of a protonation reagent and/or chromatography on a fluorous affinity resin. Methods also include separating lysed cell debris and denatured proteins/disrupted enzymes from a metabolite mixture in a container with a filter, where live cells are grown prior to the lysis, either adherent to the filter or in suspension above the filter. The cells are then lysed in the container.
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