A perfusion device and a perfusion method can allocate two routes of perfusate inflow pathways and two routes of perfusate outflow pathways for a liver graft. The perfusate inflow pathways have perfusate inflow cannulas connected respectively to the portal vein and the hepatic artery. The perfusate outflow pathways have perfusate outflow cannulas connected respectively to the suprahepatic inferior vena cava and the infrahepatic inferior vena cava. Perfusate is allowed to enter the liver from the perfusate inflow cannulas, and the perfusate in the liver is allowed to drain off from the perfusate outflow cannulas. This significantly shortens the time length of the liver graft being in an ischemic condition when the liver is removed or transplanted. That is, the onset of disorder in the liver graft can be suppressed. This increases the success rate of liver transplantation. Thus, deterioration of organ grafts during surgery is prevented in organ transplantation surgery that requires long hours.
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