首页> 外国专利> CELL CULTURE SUBSTRATUM, METHOD FOR PRODUCING CELL-CONTAINING MATERIAL, METHOD FOR PRODUCING CELL CULTURE SUBSTRATUM, METHOD FOR OBSERVING CELLS, AND CELL CULTURE SUBSTRATUM MAINTENANCE FLUID

CELL CULTURE SUBSTRATUM, METHOD FOR PRODUCING CELL-CONTAINING MATERIAL, METHOD FOR PRODUCING CELL CULTURE SUBSTRATUM, METHOD FOR OBSERVING CELLS, AND CELL CULTURE SUBSTRATUM MAINTENANCE FLUID

机译:细胞培养基质,生产含细胞材料的方法,生产细胞培养基质的方法,观察细胞的方法以及细胞培养基质维持液

摘要

The purpose of the present invention is to provide a cell culture substratum which has excellent resistance to liquid culture media and low cytotoxicity, can achieve a high cell adhesion ratio and a high viability of cultured cells, has excellent thermal stability, and is less likely to absorbs ultraviolet ray. A cell culture substratum which is provided with a substrate made from an inorganic material and has multiple concavo-convex structures on a culturing surface thereof, wherein, when the concavo-convex structures are measured with an atomic force microscope in accordance with JISB0601 and JISR1683 (measured area: a 1 μm-square, cut-off value of a low-pass contour curve filter: 1 nm, cut-off value of a high-pass contour curve filter: 170 nm), the average of the lengths of contour curve elements of the concavo-convex structures is 1 to 170 nm as measured in at least one direction (when a curve showing long-wavelength components that are blocked by the high-pass contour curve filter is converted to a straight line by the least square method, the average line is a line that is parallel with the straight line and indicates a height cumulative relative frequency distribution in the contour curve of 50%).
机译:本发明的目的是提供一种细胞培养基质,其对液体培养基具有优异的抗性并且细胞毒性低,可以实现高细胞粘附率和高培养细胞活力,具有优异的热稳定性,并且不易吸收紫外线。一种细胞培养基质,其具有由无机材料制成的基材,并且在其培养表面上具有多个凹凸结构,其中,当根据JISB0601和JISR1683用原子力显微镜测量凹凸结构时(测量面积:1μm平方,低通轮廓曲线滤光片的截止值:1 nm,高通轮廓曲线滤光片的截止值:170 nm),轮廓曲线长度的平均值至少在一个方向上测得的凹凸结构的元素为1至170 nm(当显示被高通轮廓曲线滤波器阻挡的长波分量的曲线通过最小二乘法转换为直线时) ,平均线是与直线平行的线,表示等高线中的高度累积相对频率分布为50%。

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