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METHODS AND SYSTEMS FOR IDENTIFYING CRISPR/CAS OFF-TARGET SITES
METHODS AND SYSTEMS FOR IDENTIFYING CRISPR/CAS OFF-TARGET SITES
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机译:识别CRISPR / CAS非靶位的方法和系统
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摘要
Methods and systems for searching genomes for potential CRISPR off-target sites are provided. In preferred embodiments, the methods include identifying possible on- and off-target cleavage sites and /or ranking the potential off-target sites based on the number and location of mismatches, insertions, and/or deletions in the gRNA guide sequence relative to the genomic DNA sequence at a putative target site in the genome. These methods allow for the selection of better target sites and/or experimental confirmation of off-target sites and are an improvement over partial search mechanisms that fail to locate every possible target site.
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