BYPASSING THE PAM REQUIREMENT OF THE CRISPR-CAS SYSTEM

摘要

The present CRISPR-Cas9 systems can cleave a double-stranded DNA (dsDNA) independent of the protospacer adjacent motif (PAM). By utilizing an invader RNA (iRNA) to separate at least one portion of the dsDNA, the present system and method offer great flexibility to modify a large range of DNA targets.