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Protein purification using intein-hydrophobin tag and alcohol precipitation
Protein purification using intein-hydrophobin tag and alcohol precipitation
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机译:使用内含蛋白-疏水蛋白标签和乙醇沉淀纯化蛋白质
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摘要
A method of downstream purification of a recombinant protein in fusion with a hydrophobin-intein tag using alcohol precipitation. The method comprises (1) constructing a plasmid expressing a fusion protein in a host cell, wherein the fusion protein includes a target protein domain, an intein, and a hydrophobin domain; (2) culturing the host cell transfected with the plasmid forming a cell culture medium; (3) separating a cell culture supernatant containing the fusion protein from the cell culture medium; and (4) purifying a substantial amount of the target protein from the cell culture supernatant without its hydrophobin-intein fusion tag using alcohol precipitation.
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