METABOLIC ENGINEERING OF MEMBRANE PROTEINS TO IMPROVE ESCHERICHIA COLI MEMBRANE INTEGRITY AND PRODUCTION OF FATTY ACIDS

摘要

The present disclosure provides bacteria with improved membrane integrity related primarily to outer membrane protein content and increased fatty acid production. According to the invention, Applicants have successfully demonstrated that modulation of membrane protein FADL increased membrane integrity and final fatty acid titre by 34%, Surprisingly, applicants also showed that combining the FADL regulation with a decrease or inactivation of OmpF in bacterial membranes resulted in a synergistic increase of 53% fatty acid production. The invention also provides for an isolated or recombinant bacterium, and use of the same for fatty acid production.