RECOMBINANT PLASMID pPA-OPRF-ATOX-OPRI DNA, WHICH ENCODES SYNTHESIS OF HYBRID RECOMBINANT PROTEIN, INCLUDING AMINO ACID SEQUENCES OF PROTEIN F AND I OF EXTERNAL MEMBRANE AND ATOXIC VARIANT OF A PSEUDOMONAS AERUGINOSA EXOTOXIN, ESCHERICHIA COLI PA-OPRF-ATOX-OPRI STRAIN - PRODUCER OF HYBRID RECOMBINANT PROTEIN AND METHOD OF OBTAINING SPECIFIED PROTEIN

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology and is a recombinant plasmid DNA pPA-OPRF-ATOX-OPRI, which contains fusion genes encoding proteins F and I of the outer membrane of Pseudomonas aeruginosa, as well as the toxic variant of exotoxin A Pseudomonas aeruginosa. Their expression is under the control of the promoter of phage T7, two lactose operons, lambda t₀ of region of transcription stop, transcription terminator rrnBT1. pPA-OPRF-ATOX-OPRI plasmid contains the kanamycin resistance gene, the site of replication initiation of the plasmid ColE1. Cells of the recipient Escherichia coli BL21 (DE3) strain containing the highly expressed RNA polymerase gene of bacteriophage T7 are transformed with this plasmid. Invention also relates to a method for producing a recombinant protein.;EFFECT: this invention allows to obtain a hybrid recombinant protein, including the amino acid sequence of proteins F and I of the outer membrane and the atoxic variant of exotoxin A Pseudomonas aeruginosa, to develop a vaccine to prevent infections caused by Pseudomonas aeruginosa, as well as to obtain donor immunoglobulins intended for the treatment of Pseudomonas infection.;3 cl, 3 dwg, 1 tbl, 5 ex