Super-Resolution Fluorescence Microscopy Method Using Improved Drift Compensation Markers
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机译:使用改进的漂移补偿标记的超高分辨率荧光显微镜方法
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摘要
A super-resolution fluorescence microscopy method comprises (a) providing a drift compensation marker, wherein (i) the drift compensation marker comprises a coated plasmonic particle; (ii) the drift compensation marker comprises an anisotropic plasmonic particle; and/or (iii) the drift compensation marker comprises a plasmonic particle comprising at least one reporter molecule on the surface of the plasmonic particle or on the surface of a coating; (b) performing super-resolution fluorescence microscopy; (c) using the drift compensation marker to improve the resolution of the super-resolution fluorescence microscopy. A super-resolution fluorescence microscopy method alternatively comprises (a) providing a drift compensation marker comprising a nonplasmonic Raman-active particle and wherein optionally the drift compensation marker comprises a coated nonplasmonic Raman-active particle and/or optionally the drift compensation marker comprises an anisotropic nonplasmonic Raman-active particle; (b) performing super-resolution fluorescence microscopy; (c) using the drift compensation marker to improve the resolution of the super-resolution fluorescence microscopy.
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