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TUMOR TISSUE CRYOPRESERVATION KIT, TUMOR TISSUE RESUSCITATION KIT, AND METHOD OF CRYOPRESERVING AND/OR RESUSCITATING TUMOR TISSUE

机译:肿瘤组织冷冻套件,肿瘤组织复苏套件以及冷冻和/或复苏肿瘤组织的方法

摘要

A kit, including: a first solution, a second solution, and a metal mesh. The first solution includes: dulbecco's modified eagle medium (DMEM), 65-95 V/V %; dimethyl sulfoxide (DMSO), 5.5-20 V/V %; ethylene glycol (EG), 3.5-15 V/V %; bovine serum albumin (BSA), 0.5-4 W/V %; sucrose 1-5 W/V %; methylcellulose with a viscosity of 4000 centipoise (cP), 0.05-0.8 W/V %; hetastarch 0.25-0.6 W/V %; and glucose 15-35 W/V %. The second solution includes: DMEM, 65-95 V/V %; DMSO, 5.5-20 V/V %; EG, 8-20 V/V %; BSA, 0.5-4 W/V %; sucrose, 10-20 W/V %; methylcellulose with a viscosity of 4000 centipoise (cP), 0.05-0.8 W/V %; polyvinyl pyrrolidone (PVP), 0.25-0.6 W/V %; and glucose 15-35 W/V %. The metal mesh has a thickness of 0.15-0.2 mm and includes a plurality of square holes. The side length of the square holes is 2.0-3.0 mm, and the spacing between adjacent holes is 0.5-2.0 mm.
机译:套件,包括:第一溶液,第二溶液和金属网。第一种解决方案包括:dulbecco改良的Eagle介质(DMEM),65-95 V / V%;二甲基亚砜(DMSO),5.5-20 V / V%;乙二醇(EG),3.5-15 V / V%;牛血清白蛋白(BSA),0.5-4 W / V%;蔗糖1-5 W / V%;粘度为4000厘泊(cP),0.05-0.8 W / V%的甲基纤维素; 0.25-0.6 W / V%;和葡萄糖15-35W / V%。第二种解决方案包括:DMEM,65-95 V / V%; DMSO,5.5-20 V / V%; EG,8-20 V / V%;牛血清白蛋白,0.5-4 W / V%;蔗糖,10-20 W / V%;粘度为4000厘泊(cP),0.05-0.8 W / V%的甲基纤维素;聚乙烯吡咯烷酮(PVP),0.25-0.6 W / V%;和葡萄糖15-35W / V%。金属网的厚度为0.15-0.2mm,并且包括多个方孔。方孔的边长为2.0-3.0 mm,相邻孔之间的间距为0.5-2.0 mm。

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