EmbriogÉnicos Accelerated production of Callus, embryos SOMÁTICOS and related Processing Methods

摘要

Provided is a method for the production of embryogenic Callus and Somatic embryos of soybean.It also provides methods for generating Transform explants including somatic Embryo tissue.The transgenic somatic embryos produced can be used to regenerate transgenic plants and stable.Claim 1: a method for the production of embryonic CallusThe Method comprises: selecting one or more soybean immature embryos; one or more immature embryos subjected to cold treatment; and induce embryos treated in Cold Forming Callus EMBRiogénicos.Claim 13: a method for the transformation of soybeanThe Method comprises: selecting multiple soybean immature embryos immature embryos; subject to cold treatment; transform the cigóticos immature embryos treated with coldOne or more embryos and exogenous transgenes; induce cigóticos processed to form somatic embryos and thus produce transgenic somatic Embryo tissue.