首页> 外国专利> PSCATPG PROMOTER OF SUGAR CANE AND USE OF THE SAME FOR MODULATION OF GENE TRANSCRIPTION IN PLANTS

PSCATPG PROMOTER OF SUGAR CANE AND USE OF THE SAME FOR MODULATION OF GENE TRANSCRIPTION IN PLANTS

机译:甘蔗的PSCATPG启动子及其在植物基因转录调控中的应用

摘要

sugarcane pscatpg promoter and its use for modulation of gene transcription in plants. The present invention describes a sugarcane promoter for regulating gene expression in plants, which was called the pscatpg promoter. more specifically, the technology relates to the use of the pscatpg promoter for activating nucleotide sequence expression in monocotyledon and dicotyledonous plants, especially in green tissues. the DNA fragment, nucleotide sequence comprising 1018 base pairs, was cloned upstream of the gus reporter gene in plasmid phgwfs7 and evaluated by transient transformation into sugarcane callus and benthamian nicotiana leaves. callus and leaf reporter gene activation evidenced by histochemical analysis validated pscatpg promoter activity for biotechnological purposes.
机译:甘蔗pscatpg启动子及其在植物中调节基因转录的用途。本发明描述了用于调节植物中基因表达的甘蔗启动子,称为pscatpg启动子。更具体地,该技术涉及pscatpg启动子用于激活单子叶植物和双子叶植物,特别是绿色组织中的核苷酸序列表达的用途。将该DNA片段(包含1018个碱基对的核苷酸序列)克隆到质粒phgwfs7中的gus报告基因的上游,并通过瞬时转化入甘蔗愈伤组织和本生烟草叶片中进行评估。组织化学分析证明了愈伤组织和叶片报告基因的激活,证实了pscatpg启动子活性可用于生物技术目的。

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