method for electrochemical detection of schistosoma mansoni worm genome using electroplated gold platform

摘要

The present invention is the result of a simple methodology for obtaining gold nanoparticles by direct electrodeposition of gold on the electrode surface and its application in the development of a diagnostic device focused on innovative health methodologies with high sensitivity and specificity for detection. of schistosoma mansoni. Thus, we achieved a preparation technique by self-assembling by electroplating gold nanoparticles (npsau) on the surface of the 3-mercaptopropyltrimethoxysilane modified gold electrode (mpts). Gold nanoparticles are used to immobilize thiolate DNA probes on the electrode surface due to their properties such as retaining biological activity, efficient conduction of the electrochemical signal between transducer and interface allowing amplification of electrical response and the organosilane mpts offers self-assembling gold monolayer and acts proven efficiently between the electrode surface. The techniques of cyclic voltammetry and electrochemical impedance spectroscopy were useful for monitoring electrode modification steps and observing molecular hybridization using the potassium ferricyanide redox indicator. The biosensitive system developed has the ability to detect the schistosoma mansoni genome at low concentrations and is useful for the evaluation of schistosomiasis patients.