In vitro efficacy of new synthetic benzimidazole-related compounds against Schistosoma mansoni adult worms

摘要

cqvip:Objective:To evaluate the in vitro antischistosomal activity of two new synthetic benzimidazole-related compounds:NBTP-OH and NBTP-F.Methods:Schistosoma adult worms were recovered from mice infected with Schistosoma mansoni cercaria,washed and then incubated in the culture media with different concentrations of compounds NBTP-OH and NBTP-F up to 72 h.Scanning electron microscopy was conducted to report morphological changes.Results:Incubation of adult Schistosoma mansoni with 10 μg/mL of NBTP-OH for 48 h killed 81.25% of worms.The calculated LC50and LC90 72 h post-incubation were 6.8 μg/mL and 9.8 μg/mL,respectively.Exposure of worms to 10 μg/mL of NBTP-F killed89.5% of worms after 48 h,mostly males(83.3%),the LC50 and LC90 after 72 h of incubation were 4.8 μg/mL and 6.9 μg/mL,respectively.Worms incubated for 72 h with these compounds revealed swelling and deformity of oral sucker,disorganization and erosion of the tegument when examined with scanning electron microscopy.Conclusions:NBTP-OH and NBTP-F possess in vitro antischistosomal activities;however,in vivo studies should be conducted to examine their antischistosomal effects..