methylopyl sp. and use thereof in the preparation of selective resolution of (s) -alpha-ethyl-2-oxo-1-pyrrolidine acetate

摘要

The present invention describes methyl spila sp. and use thereof in the preparation of selective resolution of (s) -alpha-ethyl-2-oxo-1-pyrrolidine acetate. methylopyl sp. which produces enzymes is subjected to cell immobilization, and is then applied to the biological resolution of an acetic acid (r, s) -alpha-ethyl-2-oxo-1-pyrrolidine ethyl ester racemate to prepare (s) ethyl ester High optical purity acetic) alpha-2-oxo-1-pyrrolidine, which is further subjected to a hydrolysis reaction to obtain (s) -alpha-ethyl-2-oxo-1-pyrrolidine acetate. The present invention achieves a high conversion yield of up to 50.0% or more, good stereoselectivity, and an enantiomeric excess value of (s) -alpha-ethyl-2-oxo-1-pyrrolidine acetic acid ethyl ester. less than 99.5; catalytic efficiency is high; The concentration of racemic substrate in the resolution reaction is up to 500 g / l, the reaction time does not exceed 15 hours, the number of reuse times of immobilized cells is not lower than 35, and thus the present invention is convenient. For industrial production, it is simple in downstream separation and produces little environmental pollution.