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METHOD FOR PRODUCING DNA PROBE AND METHOD FOR ANALYZING GENOMIC DNA USING THE DNA PROBE.

机译:生产DNA探针的方法和使用该DNA探针分析基因组DNA的方法。

摘要

This invention provides a DNA probe that is applicable to a DNA library prepared in a simple manner with excellent reproducibility. Such DNA probe is produced by a method comprising steps of performing a nucleic acid amplification reaction in a reaction solution containing genomic DNA and a random primer at a high concentration, so as to obtain a DNA fragments with the use of the genomic DNA as a template; determining the nucleotide sequence of the resulting DNA fragments; and, on the basis of the nucleotide sequence of the DNA fragments obtained in the step above, designing a DNA probe used for detecting a DNA fragment.
机译:本发明提供了一种DNA探针,该DNA探针适用于以简单的方式制备的DNA文库,具有优异的可重复性。这样的DNA探针通过以下方法制备:该方法包括以下步骤:在含有高浓度基因组DNA和随机引物的反应溶液中进行核酸扩增反应,从而以基因组DNA为模板获得DNA片段。 ;确定所得DNA片段的核苷酸序列;根据上述步骤得到的DNA片段的核苷酸序列,设计用于检测DNA片段的DNA探针。

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