Genomic DNA Extraction Method for Mulberry Varieties in Anhui Province

摘要

[Objective] The paper aimed to get a efficient,stable and low-priced DNA extraction method of mulberry leaves. [Method] With tender leaves of mulberry varieties in Anhui Province as materials,genomic DNA was extracted with CTAB method,and further identified by ultraviolet spectrophotometer,agarose gel electrophoresis and PCR methods. [Result] The A260/A280 ratio of extracted DNA was 1. 80-1. 90,and the yield was 0. 187-0. 275 μg/mg. With extracted DNA as the template,clear target bands were obtained by PCR. [Conclusion]Extracted DNA was good in quality,which could satisfy the needs of follow-up molecular biological operation.