Bispecific tetravalent antibodies with binding specificity for first and second egfr family members and methods of making and using thereof

摘要

A bispecific tetravalent antibody comprising an IgG having a pair of heavy chains and a pair of light chains, and two scFv components being connected to either C or N terminals of the heavy or light chains. The bispecific tetravalent antibody may have a binding specificity for two different members of EGFR family. In an embodiment, each scFv domain is connected to the C-terminus of either of the IgG1 heavy chains. In a further embodiment, the tetravalent antibodies described herein have a binding specificity for EGFR and HER3.