Method for producing androgenetic carrot plants in anther cultures

摘要

The notification shall cover the production of androgenetic carrot plants in pollen crops,in which the germ line B5 (Gamborgi in) is used for the induction of androgenetic carrots. 1968) without CaCl2x6H2O and with the addition of CaSiO3 in 1180 mg /l,For the regeneration of carrot plants derived from androgenetic embryos, regenerative medium B5 (Gamborgi in. 1968) without CaCl2x6H2O and with the addition of CaSiO3 in 1180 mg /l,without hormones and amino acids,with sucrose content reduced to 20 g /l,and the soil, which is a mixture of peat and sand with 1:3, is used for the acclimation of carrot plants to ex vitro conditions,nitrogen -enriched in quantities of 1,2 kg /m3,CaCOa additive in quantities of 8 kg /m3 and CaSiO3 in quantities of 2,2 g /l.