the present invention provides for a new sulfated glycosaminoglycan, on the basis of the tunics of sea cucumbers (holothuria polii) with anticoagulant activity of interest.the invention relates more particularly to the extraction, purification and structural characterization of a new anticoagulant agent, polysaccharide in nature.according to the method of the invention, the production of sulfated glycosaminoglycan is based on an enzymatic hydrolysis by papain and partially purified by a cationic detergent.the cetylpyridinium chloride (cpc), followed by fractionation, identification, characterization and evaluation of their biological activity.the purified glycosaminoglycan has been identified as a chondroitin sulfate (csfu fucosylated.the structural analysis by fourier transform infrared spectroscopy (ftir) and nuclear magnetic resonance (nmr) showed that the mono - and two-dimensional chemical structure of csfu is formed by disaccharidiques units consisting of glucuronic acid (aglu) ~ 1 / 3 of n-acetylgalactosamine (galnac) residues, most of which are variations in position c4 and c6 (galnac4,6s).these units are connected by disaccharidiques residues fucoses 2.4 - 3.4 - disulfate or disulfate.the percentage composition of the csfu reveals, in particular, a high content of sulphate (so4) groups 43.1 + 1% and a content of glucuronic acid to 22.5 + 1.9% with a 100 / carboxylate). in addition, the csfu is 60 + / - 5% of the crude polysaccharide fraction.the average molar mass number (mn) and weight (mw) of the polysaccharide were 35, 1 and 2.5 kda.in the present invention.the study of the anticoagulant activity of the purified csfu was highlighted by several specific tests of coagulation in either plasma or purified in the presence of hépatine systemcofactor ii (hcii) and antithrombin (at) in comparison with anticoagulant polysaccharides such as heparin and standard dermatan sulfate from the intestinal mucosa of swine.the csfu has remarkable anticoagulant activity mediated by physiological inhibitors of coagulation in the at and the hcii.it is extremely important to note that the csfu of sea cucumber (holothuria polii) has a pro and anticoagulant activity.according to the invention, the dual effect of coagulation is described for the first time in the native molecule.the csfu represents an alternative heparin like potential of marine origin and no adverse effects on platelet function.
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