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Method for preparing virus like particles (TSPs) derived from Plants

机译:制备植物来源的病毒样颗粒的方法

摘要

Methods for preparing virus like particles (TSPs) derived from plants. The Method may include obtaining a plant or Plant material including PSV, located in the apoplastos, produce a fraction of a fraction of esferoplastos protoplasts and / apoplastos from plant material or Plant and recover the apoplastos fraction.The fraction of the apoplastos comprises the PSV derived from plants. Alternatively, the PSV can be obtained from a plant or a Plant material including PSV from Plants by the digestion of Plant material with a composition comprising an enzyme capable of degrading the cell wall, thus producing a fraction digested.The fraction digested filtered to produce a filtered fraction derived from plants, and the PSV are recovered from the filtered fraction. Claim 32: the method of claim 29, wherein the PSV derived from Plants include influenza hemagglutinin.
机译:制备植物来源的病毒样颗粒(TSP)的方法。该方法可以包括获得位于质外体中的包括PSV的植物或植物材料,从植物材料或植物中产生一部分esferoplastos原生质体和质子体,并回收该质外体部分。来自植物。备选地,可以通过用包含能够降解细胞壁的酶的组合物消化植物材料,从植物或包括来自植物的PSV的植物材料中获得PSV,将消化的部分过滤以产生淀粉。从植物中分离得到的级分和PSV从级分中回收。权利要求32:权利要求29的方法,其中源自植物的PSV包括流感血凝素。

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