Methods to select competent oocytes and competent embryos with high pregnancy outcome potential

摘要

A method for selecting an oocyte that, when fertilized, produces a viable embryo with a high implantation rate that produces pregnancy, which comprises a stage of: a) measuring the level of expression of 45 genes in a cumulus cell surrounding said oocyte, in which said 45 genes are a member 6 of the wingless type family with an integration site for MMTV (WNT6), domain containing repetitions rich in leucine and calponin homology (HC) (LRCH4), paired box 8 (PAX8) , calcium-binding protein 4 (CABP4), GMPc-specific phosphodiesterase 5A (PDE5A), BCL2 type 11 (BCL2L11), phosphoenolpyruvate carboxykinase 1 (PCK1), transcription factor 20 (TCF20), member 6 of the SLAM family (SLAMF6 ), erythropoietin receptor (EPOR), gamma 6 subunit of the voltage-dependent calcium channel (CACNG6), NLR family containing the pyrine 1 domain (NLRP1), platelet adhesion molecule / endothelial cells (PECAM1), nitric oxide synthase 1 (US1), de facto r transcription activator 3 (ATF3), protein 8-1 associated with keratin (KRTAP8), ionotropic glutamate receptor type kainate 5 (GRIK5), member 3 of the solute transporter family 24 (SLC24A3), family member 12 of solute transporters 5 (SLC5A12), member 2 of the solute transporter family 10 (SLCA10A2), member 1A2 of the solute transporter family for organic anion transporter (SLCO1A2)), member 5 of the transporter family of solutes 25 (SLC25A5), synaptophysin type 2 (MG29), neuroligin 2 (NLGN2), alpha catalytic cAMP-dependent protein kinase (PRKACA), homologue B of the murine osteosarcoma viral oncogene FBJ (FOSB), ST3 beta-galactoside alpha-2 , 3-sialyltransferase 3 (SIAT6), lysyl oxidase type 2 (LOXL2), perforin 1 (PRF1), ADP-ribosilarginine hydrolase (ADPRH), member 3 of the beta-amyloid precursor protein binding family (A4) (APBB3) , rapid growth response gene 3 (EGR3), re cannabinoid ceptor 2 (CNR2), interferon-induced transmembrane protein 1 (IFITM1), group V phospholipase A2 (PLA2G5), calmodulin-binding transcription activator 1 (CAMTA1), SRY (sex-determining region Y) box 4 (SOX4 ), nuclear factor I / B (NFIB), nuclear factor I / C (NFIC), single-stranded interaction protein 1 with RNA binding motif (RBMS1), switch G0 / G1 2 (G0S2), homolog 3 of the tumor suppressor FAT (FAT3), member 1 of the solute transporter family 40 (SLC40A1), glypican 6 (GPC6) and insulin-like growth factor receptor 1 (IGF1R), and b) compare said level of expression measured in stage a ) with that measured in a control, said control being a cumulus cell that surrounds an oocyte that, once fertilized, has produced a viable embryo associated with a high implantation rate that causes pregnancy; and c) evaluate that said oocyte will not produce, once fertilized, a viable embryo associated with a high implantation rate that causes pregnancy, if in stage b) a differential expression between said oocyte and said control is measured.