PYRUVATE CARBOXYLASE HAVING A FEEDBACK-RESISTANT MUTATION, ENCODING DNA, PLASMIDES, AND MICROORGANISM FOR THE PRODUCTION THEREOF, AND METHODS FOR THE PRODUCTION OF PRODUCTS THE BIOSYNTHESIS OF WHICH INCLUDES OXALOACETATE AS PRECURSOR

摘要

The invention relates to pyruvate carboxylase and a pyruvate carboxylase-encoding DNA, a plasmid containing said DNA and a microorganism for the production thereof, and to methods for the production of products the biosynthesis of which includes oxaloacetate as a precursor, and to a chromosome. The invention makes a DNA available that codes for a pyruvate carboxylase having an at least 70% sequence identity with sequence no. 1 and where the triplet that codes for isoleucine-1012 is replaced by a serine-encoding triplet in positions 3034-3036. The expression of said gene results in a pyruvate carboxylase by means of which the yield of the reaction of metabolic products derived from oxaloacetate is by 7% higher with respect to the strain containing the native Pyc.