CRISPR BASED TOOL FOR CHARACTERIZING BACTERIAL SEROVAR DIVERSITY

摘要

A process for identifying Salmonella serovars present in an environmental sample includes (i) a first PCR step of amplifying polynucleotides containing one or more of CRISPR1 and CRISPR2 loci using tailed divergent primers directed to the invariant direct repeats in Salmonella CRISPR arrays, (ii) isolating and purifying the products of step (i), (iii) a second PCR amplification step of amplifying the purified products from the second step, (iv) isolating and purifying the products of step (iii), (v) sequencing the purified products of the prior step and using the sequences to identify discrete serovars in the sample.