Primer set for amplifying multiple target DNA sequences in sample and use thereof

摘要

Provided are a primer set for amplifying multiple target DNA sequences in a sample and a method for amplifying multiple target DNA sequences in a sample using the primer set. The primer set comprises multiple pairs of upstream and downstream specific primers for each target DNA sequence. Each pair of upstream and downstream specific primers comprises specific sequences for the target sequence, and the specific sequences satisfy with the following conditions: (1) no amplification occurs between the specific sequences and sequences other than the target sequence; (2) no dimer forms between the specific sequences; and (3) no hairpin structure forms. A consensus sequence that is not homologous to the genome is linked to the 5' terminus of the specific sequence. The 3' terminus of the specific sequence has a modification for increasing the steric hindrance, and the modification does not hinder the specific sequence from binding to a perfectly matching template with the specific sequence and elongating, but basically hinders the specific sequence from binding to an imperfect matching template and elongating.