首页> 外国专利> DESIGN OF PROTEIN GLYCOSYLATION SITES BY RAPID EXPRESSION AND CHARACTERIZATION OF N-GLYCOSYLTRANSFERASES

DESIGN OF PROTEIN GLYCOSYLATION SITES BY RAPID EXPRESSION AND CHARACTERIZATION OF N-GLYCOSYLTRANSFERASES

机译：N-糖基转移酶的快速表达和表征设计蛋白质糖基化位点

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摘要

Disclosed are components, systems, and methods for glycoprotein or recombinant glycoprotein protein synthesis in vitro and in vivo. In particular, the present invention relates to components, systems, and methods for identifying amino acid glycosylation tag motifs for N-glycosyltransferases and the use of the identified amino acid glycosylation tag motifs in methods for preparing glycoproteins and recombinant glycoproteins in vitro and in vivo.

机译：公开了体外和体内的糖蛋白或重组糖蛋白蛋白合成的成分，系统和方法。特别地，本发明涉及用于鉴定N-糖基转移酶的氨基酸糖基化标签基序的组分，系统和方法，以及所鉴定的氨基酸糖基化标签基序在制备糖蛋白和重组糖蛋白的方法中的用途。 / I>和体内。 展开▼

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公开/公告号WO2019035916A1

专利类型

公开/公告日2019-02-21

原文格式PDF

申请/专利权人 NORTHWESTERN UNIVERSITY;
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申请/专利号WO2018US00185

发明设计人 JEWETT MICHAEL CHRISTOPHER;KIGHTLINGER WESTON K.;LIN LIANG;MRKSICH MILAN;
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申请日2018-08-15

分类号C12P19/18;C12N9/10;C07K19;C12P21;

国家 WO

入库时间 2022-08-21 11:56:29

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1. 通过用内切H共表达的体内N-去糖基化重组蛋白质的生产 [P] . 中国专利： CN108463550A . 2018-08-28

2. 过表达N-糖基化途径调节基因以调节重组蛋白的糖基化 [P] . 中国专利： CN106170554B . 2021.08.17

3. Modifying glycosylation profile of a glycosylation site contained in a target peptide, comprises recombinantly producing a fusion protein made of a mint protein and the target peptide, which contains predetermined glycosylation site [P] . 德国专利： DE102012105416A1 . 2013-12-24

机译：修饰靶标肽中包含的糖基化位点的糖基化分布包括重组产生由薄荷蛋白和靶标肽组成的融合蛋白，所述融合蛋白包含预定的糖基化位点

4. Expression and secretion of heterologous proteins in yeast - using truncated a-factor leader sequence contg. signal peptide and glycosylation site [P] . DK176075B1 . 2006-04-10

机译：使用截断的a因子前导序列contg在酵母中表达和分泌异源蛋白。信号肽和糖基化位点

5. Overexpression of N-glycosylation pathway regulators to regulate glycosylation of recombinant proteins [P] . JP2021040638A . 2021-03-18

机译： N-糖基化途径调节剂的过度表达调节重组蛋白的糖基化

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