CONSTRUCTION OF CD27 EUKARYOTIC EXPRESSION VECTOR AND PREPARATION OF CELL STRAIN HIGHLY EXPRESSING CD27

摘要

Provided are construction of a eukaryotic expression vector and a method for preparing a stable-expression cell strain using the vector. Specifically, the method comprises cloning a CD27 full-length gene into a pEGFP-N1 eukaryotic expression vector carrying a fluorescent protein tag, and transfecting Hela cells using the constructed eukaryotic expression vector to establish a HeLa cell line stably overexpressing CD27.