CONSTRUCTION OF FH3 EUKARYOTIC EXPRESSION VECTOR AND PREPARATION OF HIGH-EXPRESSION CELL STRAIN BY USING SAME

摘要

Provided are construction of an eukaryotic expression vector and preparation of a stable-expression cell strain by using same. The method comprises: cloning an FH3 full-length gene to a pEGFP-N1 eukaryotic expression vector with a fluorescent protein tag; transfecting HAP-s cells by using the constructed eukaryotic expression vector; and establishing an FH3 stable-overexpression HAP-s cell line.