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Synthesis of backbone modified morpholino oligonucleotides and chimeras using phosphoramidite chemistry
Synthesis of backbone modified morpholino oligonucleotides and chimeras using phosphoramidite chemistry
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机译:使用亚磷酰胺化学合成骨架修饰的吗啉代寡核苷酸和嵌合体
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摘要
Amine substituted morpholino oligonucleotides, other than the classical N,N-dimethylamino PMO analogue, and methods of efficiently synthesizing these oligonucleotides with high yield are provided. Morpholino oligonucleotides having thiophosphoramidate, phosphoramidate, phosphoramidate diester, and alkyl phosphoramidate linkers. Chimeras containing unmodified DNA/RNA and other analogs of DNA/RNA can be prepared. These oligonucleotides form duplexes with complementary DNA or RNA that are more stable than natural DNA or DNA/RNA complexes, are active with RNAse H1, and may be transfected into cells using standard lipid reagents. These analogues are therefore useful for numerous applications.
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