Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells

摘要

#$%^&*AU2018279009A120190117.pdf#####ABSTRACT The present invention provides methods, cell cultures and differentiation media to promote differentiation of pluripotent stem cells to pancreatic endocrine cells expressing PDX1, NKX6.1, and HB9 by culturing in a culture vessel at the air-liquid interface. The invention also provides for in vivo maturation of cells cultured at the air-liquid interface.