Deer Placenta Active Protein as well as Its Extraction Method and Application

摘要

#$%^&*AU2019100414A420190530.pdf#####ABSTRACT The invention discloses a deer placenta active protein as well as its extraction method and application, wherein the method comprises the following steps: Take the deer placenta to perform the pretreatment; mince the pre-treated deer placenta after freezing, add saline solution containing 5 protease inhibitor to make homogenate; immediately dissolve that homogenate after it is completely frozen, repeat for 1-3 times; ultra-sonication is performed on the treated homogenate to break up the cells, and then collect the supernatant by centrifugation; add acetone after the supernatant is cooled and collect precipitate by filtration and dry precipitate to obtain the powder; dissolve the power in a phosphate buffer solution; add saturated ammonium sulfate to a saturation of 0.25, remove the 0 precipitate by centrifugation, adjust the pH with supernatant, and then add saturated ammonium sulfate to a saturation of 0.50 to obtain salting-out; dissolve the salting-out in Tris-Hcl buffer solution and ultrafiltrate is obtained by ultrafiltration; after desalting the ultrafiltrate, it is dried to obtain the deer placenta active protein. The deer placenta active protein of the invention can promote the proliferation of stem cells. 5 12