PURITY ANALYSIS METHOD FOR EXTRACELLULAR VESICLES, USING SIZE EXCLUSION CHROMATOGRAPHY

摘要

The present invention relates to a purity analysis method for extracellular vesicles, using size exclusion chromatography, and more specifically the present invention relates to a method of using the size-specific separation ability of size exclusion chromatography and spectroscopy, that is single or in which two or more kinds are combined, in order to quantify, from various samples, the volume of extracellular vesicles and various contaminants, simultaneously, and thereby analyze the purity, total amount and yield of extracellular vesicles. The purity analysis method for extracellular vesicles, according to the present invention, has the benefit of being capable of effective analysis whilst preserving the form or properties of the extracellular vesicles. Also, the purity analysis method for extracellular vesicles, according to the present invention, makes it possible to rapidly determine, simultaneously, the purity, total amount and yield of extracellular vesicles that have been separated using a conventional method, and can be utilized in, for example, disease diagnosis, disease treatment, multi-omics research and research into the properties of extracellular vesicles which use separated extracellular vesicles. In addition, the purity analysis method according to the present invention can also be applied to the development of a new separation method.