Base on the principle of absolute quantification of size exclusion chromatography(SEC),a light scattering(LS) detector coupled with a concentration detector(refractive index detector) is utilized to determine the compositions of complicated binary mixtures.A theoretical analysis predicts that the response factors for both LS and RI detectors are linear functions with the composition of any specified polymer mixtures in the binary polymer mixtures.Two pairs of complicated binary mixtures were used to test the theory mentioned in the present paper,and the experimental results show an excellent accordance with the theory.
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机译:Rapid determination of indapamide in human urine using novel low-density solvent based ultrasound assisted emulsification microextraction coupled with high performance liquid chromatography-variable wavelength detection