A METHOD FOR PRODUCTION OF A PROTEIN HAVING PEPTIDYLGLYCINE ALPHA-HYDROXYLATING MONOOXYGENASE ACTIVITY

摘要

The present invention provides a method for production of a protein having peptidylglycme alpha-hydroxylating monooxygenase (PHM) activity using a coryneform bacterium having an ability to produce a protein by secretory production, which method includes steps of culturing the bacterium, which has been modified so that the nucleic acids encoding the domains CuA and CuB of the PHM are co-expressed on separate genetic constructs in the bacterium, in a culture medium to produce the protein, and collecting the produced protein from the bacterium and / or the medium. The novel technique allows for improved production of the protein having an activity of a heterologous PHM native to various species using a coryneform bacterium such as, for example, a bacterium belonging to the genera Corynebactenum and Brevibacterium.