SYNTHESIS METHOD OF POSITRON-EMITTING NUCLIDE-LABELED PROTEIN

摘要

This method uses a positron-emitting nuclide-labeled amino acid or a derivative thereof to synthesize a positron-emitting nuclide-labeled protein by means of a cell-free protein synthesis system. Said cell-free protein synthesis system is a system reconfigured by means of a factor involved in protein synthesis, and said method uses a positron-emitting nuclide-labeled unnatural amino acid as the positron-emitting nuclide-labeled amino acid or derivative thereof; the method further uses a template nucleic acid into which a stop codon has been introduced, tRNA which recognizes the introduced stop codon and binds to the positron-emitting nuclide-labeled unnatural amino acid, and an aminoacyl-tRNA synthetic enzyme which causes bonding between the tRNA and the positron-emitting nuclide-labeled unnatural amino acid.