PREPARATION METHOD FOR ENZYME-MODIFIED STEVIOSIDE, ENZYME FOR SAID PREPARATION METHOD, AND APPLICATION THEREOF

摘要

A preparation method for enzyme-modified stevioside is provided in the invention and comprises: adding beta-fructofuranosidase into a solution having a stevioside raw material and sucrose dissolved therein so as to obtain a reaction liquid, adjusting the pH of said reaction liquid to 5.0-8.0, maintaining the reaction temperature at 20-45°C, and, after stirring and reacting, collecting the enzyme-modified stevioside, the stevioside raw material comprising one or more of stevioside and rebaudioside A, and the beta-fructofuranosidase being derived from microbacterium saccharophilum or aspergillus japonicus. The preparation method is highly effective and simple, low in cost, requires little time, has a high conversion rate, is environmentally friendly, and may be widely applied in industrial scale production. The enzyme for said preparation method and the application thereof are also provided in the present invention.