L-AMINO ACID DEAMINASE MUTANTS HAVING INCREASED THERMAL STABILITY AND CONSTRUCTION METHOD THEREFOR

摘要

The present invention relates to the field of biotechnology, and provides L-amino acid deaminase mutants D340N and L363N having increased thermal stability and a construction method therefor. Compared with wild-type L-amino acid deaminase, Vmax and t1/2 of the mutants D340N and L363N are increased. The mutants can be used for catalyzing L-amino acid deamination to synthesize corresponding α-keto acid, and can be used as a catalyst for chirally-separated amino acids and derivatives thereof so as to realize separation of DL-amino acid enantiomers.