TISSUE-SPECIFIC HUMAN BIOINKS FOR THE PHYSIOLOGICAL 3D-BIOPRINTING OF HUMAN TISSUES FOR IN VITRO CULTURE AND TRANSPLANTATION

摘要

The present invention relies on the discovery that the combination of two biomaterials, human tissue-specific ECM (extracellular matrix)and a cellulose-based bioink, with or without cells, in the 3Dbioprinting of human tissues and scaffolds, results in excellent printability and improved cell function, viability and engraftment. The present invention is based on the first report describing the bioprinting of human tissue, specifically hepatic tissue,using human ECM, specifically liver ECM,in combination with cellulose-based bioink. More specifically, the present invention refers to a composition for use in 3Dbioprinting and culturing of human or animal tissue comprising(i)cellulose nanofibril-based bioink, and(ii) human or animal tissue-specific extracellular matrix (ECM) material. Further, the invention relates to a method for 3Dbioprinting of human tissue and /or scaffolds comprising bioprinting the composition of the invention, as well as the bioprinted tissues and/or scaffolds. Moreover, the invention relates to various applications using the bioprinted tissues and/or scaffolds.