METHOD FOR INCREASING EFFICIENCY OF HOMOLOGOUS RECOMBINATION-BASED GENE EDITING IN PLANT

摘要

The present invention relates to a method for increasing the efficiency of homologous recombination-based gene editing in a plant, comprising a step of optimizing temperature and photoperiod conditions, expressing factors necessary for homology-directed DNA repair (HDR) and factors for increasing the efficiency of HDR by using multiple replicons, or regulating an HDR pathway or a non-homologous end joining (NHEJ) pathway, during tissue culturing of plant cells.