HIGH-FREQUENCY SOMATIC EMBRYO REGENERATION GROWTH MEDIUM WITHOUT GERMPLASM GENOTYPE RESTRICTION AND APPLICATION THEREOF

摘要

Disclosed by the present invention is a somatic embryo induction growth medium, comprising a modified MS growth medium, 2-4mg/L2, 4-D, 0.2mg/L NAA, 0.1mg/L6-BA, 40g/L saccharose, and 0.7% agar, having a pH value of 5.8-6.0. Also disclosed by the present invention are an embryoid proliferation growth synthetic growth medium and a high-frequency somatic embryo regeneration growth medium without a germplasm genotype restriction. Also disclosed by the present invention are the somatic embryo induction growth medium and an application of the synthetic growth medium. In the present invention, there is excellent coordination of such factors as: selection of a suitable explant, and prompt adjustment of the explant, the growth medium formula, the hormone component concentration, and the corresponding growth conditions on the basis of key developmental stages. This effectively overcomes the problem of different species (breeds) and genotype restrictions and the problem of high-frequency somatic embryo regeneration when culturing Zoysia japonica, significantly increasing the frequency of somatic embryogenesis and the frequency of plant regeneration.