METHOD AND DEVICE FOR MANIPULATING A BEAM PATH IN A MICROSCOPE, METHOD FOR RECORDING IMAGE STACKS IN A MICROSCOPE

摘要

The invention relates to a method for manipulating at least one beam path (8) in a microscope (1), in particular in a light sheet microscope (5), a method for recording image stacks (224) in a microscope (1), in particular in a light sheet microscope (5), a device for manipulating at least one beam path (8) in a microscope (1), in particular in a light sheet microscope (5) and a non-volatile computer-readable storage medium (163). The solutions from the prior art have the disadvantage that specimens (21) cannot be viewed with every immersion medium (23) and the methods are additionally limited to optical arrangements (9) having a low numerical aperture (NA). The method according to the invention improves solutions from the prior art by the following method steps: - determining the refractive index (n) of a specimen (21) arranged in a specimen volume (17) and/or an optical medium (35) arranged in a specimen volume (17); and - adjusting at least one microscope parameter (2) according to the determined refractive index (n) for manipulating the beam path (8).