Identification of ligands by co-fractionation

摘要

The present invention relates to a method for determining a ligand of a macromolecule, said method comprising the steps of: (a) contacting a sample comprising (i) a complex formed by said macromolecule with said ligand and (ii) To a method for separating said complex from said complex; (b) releasing a ligand from the complex obtained in step (a); And (c) the released ligand obtained in step (b) is applied to a chemical assay to determine the ligand of the macromolecule.