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DNA probe for in-situ hybridization to chromosome
DNA probe for in-situ hybridization to chromosome
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机译:用于与染色体原位杂交的DNA探针
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摘要
Kits for detection of chromosomal abnormalities, probe mixtures and probes are provided. A genetic probe is obtained by a method comprising the steps of: (a) irradiating a genetic segment for a sequence region with a non-repetitive nucleic acid sequence and selecting one or more nucleic acid sequences; (b) designing and synthesizing a primer pair for a polymerase chain reaction to a non-repeated nucleic acid sequence, wherein each of the primers comprises an oligonucleotide sequence complementary to a complementary strand of the non-repeated nucleic acid sequence or a strand thereof, Having a universal linker sequence; (c) performing a first PCR and obtaining a first mixture of nucleic acid fragments (pool A); (pool B) was performed using a chromogenic in situ hybridization or a fluorescent in situ hybridization (FISH) hybridization (hybridization) using a primer which hybridizes the mixture (pool A) / CISH / ISH). / RTI
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