A method of culturing a cell, a method of removing a suspended cell, and a method of killing a suspended cell

摘要

The present invention relates to a method of culturing a cell, comprising the steps of (1) applying a cell culture module to a first medium containing suspended cells, (2) maintaining the cell culture at a temperature at which the cells can be cultured, And (3) culturing the cell culture module on which the cells have been adsorbed, in a culture medium in a second culture medium, wherein the polymer porous membrane is a surface layer A having a plurality of pores And a macroboid layer sandwiched between the surface layer A and the surface layer B, wherein the average pore diameter of the pores existing in the surface layer A is the average pore diameter of the pores existing in the surface layer B And the macroboid layer has a plurality of macroboids bounded by the surface layers A and B and a plurality of macroboids surrounded by the partition layers and the surface layers A and B , A hole in the surface layer A and B provides a method wherein the communicating with the macrovoids.