A lot of studies on low-temperature plasma application have produced impressive results including an antibiotic effect and an anticancer effect. Although the studies on low-temperature plasma are expanded into various areas including clinical application, very little is known of the effects and action mechanism of low-temperature plasm on wound care. Inventors in the present invention have studied positive effects of growth factors induced after exposure to low-temperature plasma, and have identified that expression of the growth factors, mediated by HIF1α expression induced by low-temperature plasma, increases in primary cultured person dermal fibroblasts. In cell viability analysis, the viability of fibroblasts was reduced only in a sample which had been left for 6 hours or 24 hours after being processed with low-temperature plasma for 5 minutes. On the other hand, when being preprocessed with NAC, that is, a reactive oxygen species remover, the loss of cells was suppressed. The movement of fibroblasts, after low-temperature plasma processing, significantly increased at 6 hours and 24 hours in a scratch wound care analysis method, and cytokine expression also significantly changed while a regulating growth factor was induced after 6 hours and 24 hours. This experiment was conducted by using real-time PCR and ELISA. In particular, low-temperature plasma processing significantly induced HIF1α expression, which is a blood vessel generating up-regulator. When being preprocessed with CAY10585, HIF1α expression was hindered and production of a blood vessel generating growth factor induced by low-temperature plasma was hindered. To sum up, low-temperature plasma processing is to treat a wound by a molecular mechanism which promotes generation of blood vessels.
展开▼
