MmMVK- 3 Crystal structure of MmMVK-mevalonate complex and mevalonate kinase mutants resistant to substrate inhibition

摘要

The present invention relates to a 3D crystal structure of MmMVK-mevalonate composite and a substrate inhibition resistant mutant mevalonate kinase (MmMVK). The inventor of the present invention crystal-analyzed a 3D structure of archaea evalonate kinase proteins and mevalonate composite, a substrate, by an X-ray crystallography. In the 3D structure, a second substrate binding site different from currently known substrate binding site is found. When amino acid residue of the novel binding site is mutated, a mutant protein having a resistance to a self-inhibition for inhibiting enzyme activities according to a substrate concentration is found. The present invention determines an enzyme activity analysis of mutant proteins and a 3D structure. A newly developed mutant mevalonate kinase protein would help improve isoprene biosynthesis yield as an enzyme of rate determining step of isoprene biosynthesis in a mevalonate pathway, an isoprene biosynthesis pathway.