Ciguatoxin A subunit operon polypeptides, ciguatoxin activator scaffolds, and cell-targeting molecules for site-specific conjugation

摘要

The present invention relates to the use of molecules for site-specific positions (and sometimes unique amino acid residues of a molecule) for binding to other molecules while maintaining cigarette toxin functions such as, for example, efficient intracellular routing and / or robust cytotoxicity Scaffold and cell-targeting molecules derived from a ciguatotoxin A subunit, including amino acid substitutions that result in an amino acid substitution. The invention also relates to a cell-targeting molecule comprising site-specific positions for binding to other molecules such as, for example, cell-targeting molecules or agents that alter the properties of an organism for delivery, and / Lt; / RTI Certain molecules comprising the polypeptides of the present invention exhibit reduced immunogenicity and / or are more resistant to mammals. The cell-targeting molecules and compositions thereof of the present invention have utility as diagnostic and / or therapeutic molecules for the treatment of various diseases, disorders and conditions, for example, for the selective delivery of cargo to target-expressing cells, This includes genetic disorders, genetic predisposition, infection, cancer, tumors, growth disorders, and / or immune disorders.