CRM197 CRM197 RECOVERY AND PURIFICATION METHOD OF BIOACTIVE CRM197 PROTEINS FROM INCLUSION BODIES INCLUDING CRM197

摘要

The present invention relates to a method for expressing and purifying insoluble proteins of CRM197. By solubilizing a CRM197 protein inclusion body using a non-modified solubilizing agent, it is possible to produce an active CRM197 protein with high yield. The mild solubilization method uses Tris-Cl buffer, DMSO, n-propanol or sarkosyl solution.