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Transformation method of Solanum nigrum and Transgenic Solanum nigrum

机译:龙葵和转基因龙葵的转化方法

摘要

The present specification discloses a method for transforming Solanum nigrum, comprising the steps of: preparing Agrobacterium including a vector; culturing and growing cotyledons of Solanum nigrum in MS medium; co-culturing Agrobacterium with the cotyledons of Solanum nigrum to obtain transgenic cotyledons of Solanum nigrum; making the transgenic cotyledons of Solanum nigrum grow in a selected medium and culturing in darkness; and culturing the cotyledons of Solanum nigrum cultured in the darkness with light to obtain shoots of Solanum nigrum. The present invention also discloses a transgenic Solanum nigrum plant obtained by the transformation method of the present invention.
机译:本说明书公开了一种转化龙葵的方法,该方法包括以下步骤:制备包括载体的农杆菌;以及将农杆菌制备成载体。在MS培养基中培养和生长龙葵子叶;将农杆菌与龙葵子叶共培养以获得黑龙葵的转基因子叶;使龙葵的转基因子叶在选定的培养基中生长并在黑暗中培养;培养在黑暗中培养的龙葵子叶,获得龙葵芽。本发明还公开了通过本发明的转化方法获得的转基因龙葵。

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