Biosynthesis Preparation of Steviol Glycoside Rebaudioside D4 from Rebaudioside E

摘要

In some embodiments, the invention relates to the preparation of steviol glycoside rebaudioside D4 through the use of rebaudioside E. In some embodiments, the present invention relates to mutant CP1 enzymes, mutant HV1 enzymes, as well as host cells and methods using such enzymes, such as to prepare rebaudioside D4.